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【dmem培养基】DMEM培养基培养什么细胞可以培养F81嘛

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解决时间 2021-01-31 23:19
【dmem培养基】DMEM培养基培养什么细胞可以培养F81嘛
最佳答案
【答案】 可以养很多细胞的,DMEM(意思就是双倍(double) 盐的 MEM)比MEM营养更丰富,DMEM又分为好多种的.
  常用细胞所用培养基配方
  CELL LINE CELL TYPE SPECIES TISSUE MEDIUM
  293 fibroblast human embryonic kidney MEM,10% HI horse serum
  3T6 fibroblast mouse embryo DMEM,10% FBS
  A549 epithelial human lung carcinoma F-12K,10% FBS
  A9 fibroblast mouse connective tissue DMEM,10% FBS
  AtT-20 epithelial mouse pituitary tumor F-10,15% horse serum and 2.5% FBS
  BALB/3T3 fibroblast mouse embryo DMEM,10% FBS
  BHK-21 fibroblast hamster kidney GMEM,10% FBS or MEM,10% FBS and NEAA
  BHL-100 epitheliall human breast McCoy'5A,10% FBS
  BT fibroblast bovine turbinate cells MEM,10% FBS and NEAA
  Caco-2 epithelial human colon adeno carcinoma MEM,20% FBS and NEAA
  Chang epithelial human liver BME,10% calf serum
  CHO-K1 epithelial hamster ovary F-12,10% FBS
  Clone 9 epithelial rat liver F-12K,10% FBS
  Clone M-3 epithelial mouse melanoma F-10,15% horse serum and 2.5% FBS
  COS-1 fibroblast monkey kidney DMEM,10% FBS
  COS-3 fibroblast monkey kidney DMEM,10% FBS
  COS-7 fibroblast monkey kidney DMEM,10% FBS
  CRFK epithelial cat kidney MEM,10% FBS and NEAA
  CV-1 fibroblast monkey kidney MEM,10% FBS
  D-17 epithelial dog osteosarcoma MEM,10% FBS and NEAA
  Daudi lymphoblast human blood from a lymphoma patient RPMI-1640,10% FBS
  GH1 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
  GH3 epithelial rat pituitary tumor F-10,15% horse serum and 2.5% FBS
  H9 lymphoblast human T-cell lymphoma RPMI-1640,20% FBS
  HaK epithelial hamster kidney BME,10% calf serum
  HCT-15 epithelial human colorectal adenocarcinoma RPMI-1640,10% FBS
  HeLa epithelial human cervix carcinoma MEM,10% FBS and NEAA (in suspension,S-MEM)
  HEp-2 epithelial human larynx carcinoma MEM,10% FBS
  HL-60 lymphoblast human promyeolocytic leukemia RPMI-1640,20% FBS
  HT-1080 epithelial human fibrosarcoma MEM,10% HI FBS and NEAA
  HT-29 epithelial human colon adenocarcinoma McCoy's 5A,10% FBS
  HUVEC endothelial human umbilical cord F-12K,10% FBS and heparinat 100 ug ml
  I-10 epithelial mouse testicular tumor F-10,15% horse serum and 2.5% FBS
  IM-9 lymphoblast human marrow from myeloma patient RPMI-1640,10% FBS
  JEG-2 epithelial human choriocarcinoma MEM,10% FBS
  Jensen fibroblast rat sarcoma McCoy's 5A,5% FBS
  Jurkat lyphoblast human lymphoma RPMI-1640,10% FBS
  K-562 lymphoblast human myelogenous leukemia RPMI-1640,10% FBS
  KB epithelial human oral carcinoma MEM,10% FBS and NEAA
  KG-1 myeloblast human marrow from erythroleukemia patient IMDM,20% FBS
  L2 epithelial rat lung F-12K,10%FBS
  L6 rat skeletal muscle myoblasts DMEM,10% FBS
  LLC-WRC 256 epithelial rat carcinoma Medium 199,5% horse serum
  McCoy fibroblast mouse unknown MEM,10% FBS
  MCF7 epithelial human breast adenocarcinoma MEM,10% FBS NEAA,10ug/ml insulin
  WEHI-3b macrophage-like mouse myelomonocyte leukemia DMEM,10% FBS
  WI-38 epithelial human embryonic lung BME,10% FBS
  WISH epithelial human amnion BME,10% FBS
  WS1 human embryonic skin MEM,10% FBS and NEAA
  XC epithelial rat sarcoma MEM,10% FBS and NEAA
  Y-1 epithelial mouse tumor of adrenal F-10,15% horse serum and 2.5% FBS
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