0.1M，pH8.0的硼酸缓冲液怎么配制
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解决时间 2021-11-11 10:06
- 提问者网友：呐年旧曙光
- 2021-11-10 20:44
0.1M，pH8.0的硼酸缓冲液怎么配制
最佳答案
- 五星知识达人网友：神鬼未生
- 2021-11-10 22:15
直接用0.1M硼酸溶液滴加氢氧化钠到pH8.0 这样： SODIUM BORATE BUFFER 50x Stock:20 g NaOH pH to 8.0 with approx 120 g H3BO3 (boric acid powder) bring to 1 L with dH2O NOTE:This stock will precipitate over time,requiring heat to re-dissolve crystallized salts before dilution. 1x Working Buffer:20 ml of 50x stock 980 ml dH2O NOTE:The pH of the working solution will be higher than that of the 50x stock NOTE:Do not re-use 1x solution for more than a day or it will go fuzzy.Really. Translated to concentrations: 50x Stock:500 mM NaOH,pHed to 8.0 w/ H3BO3 1x Working buffer:10 mM NaOH,pHed to 8.5 w/ H3BO3
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