FG12质粒 和FUGW质粒是同一个质粒吗?如果有区别,区别在哪里?
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解决时间 2021-03-12 04:20
- 提问者网友:佞臣
- 2021-03-11 18:24
FG12质粒 和FUGW质粒是同一个质粒吗?如果有区别,区别在哪里?
最佳答案
- 五星知识达人网友:冷風如刀
- 2021-03-11 18:43
FG12 was derived from FUGW. The extra nucleotides from the HindIII site downstream of the Ubiquitin-C promoter (UbiC) promoter to the NcoI site in front of the initiation codon of EGFP were deleted by a HindIII-NcoI adapter ligation. Further, XbaI, EcoRI, and XhoI sites at the 3' end of EGFP and WRE were eliminated, followed by a polylinker oligonucleotide ligation at the PacI site between the Flap element and the UbiC promoter, to generate a set of new restriction sites, XbaI-HpaI-XhoI-BstXI-PacI, that is optimal for accommodating the siRNA expression cassette. To construct the siRNA-expressing lentiviral vectors, the siRNA expression cassette can be subcloned into FG12 between the XbaI and XhoI sites.
所以这两个质粒基本骨架一样,只是多克隆位点有所不同追问您好,请问可以加我好友吗?我的QQ305362173,目前有一些问题需要请教。参考资料:http://www.ncbi.nlm.nih.gov/pubmed/12518064
所以这两个质粒基本骨架一样,只是多克隆位点有所不同追问您好,请问可以加我好友吗?我的QQ305362173,目前有一些问题需要请教。参考资料:http://www.ncbi.nlm.nih.gov/pubmed/12518064
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