蛋白电泳胶的配制中，Buffer的PH值是不是会影响到样品在跑的时候连成一条线？

就是样品跑到浓缩胶大概三分之一时溴酚蓝都混一起了，看上去好像蛋白也没在各自的泳道跑也都混一起了

It does not matter that 溴酚蓝都混一起了, 溴酚蓝都混一起does not mean proteins mixing together. You have to stain the gel to find out whether proteins come together.

Buffer pH definitely is important.

也许是的。