帮我查一下这个美国专利号U.S.patent:5,382,714谢谢！

帮我查一下这个美国专利号U.S.patent:5,382,714谢谢！

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　　United States Patent 5,382,714
　　Khachik January 17, 1995

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　　Process for isolation, purification, and recrystallization of lutein from saponified marigold oleoresin and uses thereof

　　Abstract
　　A method of isolating, purifying and recrystallizing substantially pure lutein, preferably from saponified marigold oleoresin in its pure free form, apart from chemical impurities and other carotenoids. Lutein may be used as an analytical standard or in cancer prevention trials and as a safe and effective color additive for human food.

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　　Inventors: Khachik; Frederick (Beltsville, MD)
　　Assignee: The Catholic University of America (Washington, DC)

　　Appl. No.: 08/210,009
　　Filed: March 17, 1994

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　　Current U.S. Class: 568/834 ; 568/816; 568/822; 568/823; 568/824; 568/832
　　Current International Class: C07C 35/00 (20060101); C07C 35/21 (20060101); C07C 035/08 (); C07C 035/18 ()
　　Field of Search: 568/810,816,819,700,822,823,824,832,834,913 435/67 426/540,310

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　　References Cited [Referenced By]

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　　U.S. Patent Documents

　　2527602 October 1950 Wall
　　3732214 May 1973 Surmatis et al.
　　3879424 April 1975 Surmatis et al.
　　4028217 June 1977 Okada et al.
　　4048203 September 1977 Philip
　　4316917 February 1982 Antoshkiw et al.
　　4851339 July 1989 Hills
　　4871551 October 1989 Spencer
　　5019668 May 1991 Keat et al.

　　Foreign Patent Documents

　　0096032 Jun., 1983 JP

　　Primary Examiner: Lone; Werren B.
　　Attorney, Agent or Firm: Zarley, McKee, Thomte, Voorhees & Sease

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　　Claims

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　　What is claimed is:

　　1. The carotenoid composition consisting essentially of substantially pure lutein crystals derived from plant extracts that contain lutein, said lutein crystals being of the formula: ##STR2## wherein the lutein is substantially free from other carotenoids and chemical impurities found in the natural form of lutein in the plant extract.

　　2. The lutein carotenoid composition of claim 1 wherein the plant extract is derived from naturally occurring plants selected from the group consisting of fruits, vegetables and marigolds.

　　3. The lutein carotenoid composition of claim 1 admixed with an edible triglyceride oil to provide a dose form.

　　4. The lutein carotenoid composition of claim 1 wherein the lutein is derived from marigold flower extract.

　　5. A method of obtaining substantially pure, free form lutein crystals from saponified marigold extract, comprising the steps of:

　　(a) mixing the marigold extract with water/alcohol mixture remove soluble impurities;

　　(b) lowering the temperature of the mixture to a temperature to precipitate lutein crystals;

　　(c) washing the lutein crystals with water to remove water soluble impurities;

　　(d) washing the lutein crystals with an organic solvent mixture to remove organic impurities, and thereafter recrystallizing the lutein.

　　6. The process of claim 5 wherein the recrystallization involves dissolving the lutein crystals in a binary solvent system and thereafter lowering the temperature to recrystallize lutein in substantially pure form free from other carotenoids and chemical impurities.

　　7. The process of claim 6 wherein the binary solvent system is a halogenated organic solvent in which lutein is strongly soluble followed by addition of a second organic solvent in which lutein is only partially soluble.

　　8. The method of claim 7 wherein the halogenated solvent is dichloromethane, chloroform, or 1, 2-dichloroethane.

　　9. The method of claim 8 wherein the halogenated solvent contains a small but effective amount of an organic base to neutralize trace amounts of acids that are normally present in halogenated solvents.

　　10. The process of claim 9 wherein the organic base is triethylamine.

　　11. The process of claim 10 wherein triethylamine is present at about 1% by volume of the added halogenated solvent.

　　12. The process of claim 7 wherein the organic solvent is a straight chain hydrocarbon.

　　13. The method of claim 5 wherein the ratio of water to alcohol is 2.3 to 1 in step (a).

　　14. The method of claim 5 wherein the alcohol in step (a) is selected from the group consisting of ethanol, methanol and isopropyl alcohol.

　　15. The method of claim 6 wherein the temperature is lowered within the range of from about 5.degree. C. to about 10.degree. C.

　　16. The method of claim 5 wherein the organic solvent mixture in step (d) includes a straight chain hydrocarbon solvent and an alcohol.

　　17. The method of claim 16 wherein the temperature of the alcohol and the organic solvent is from about 0.degree. C. to about 5.degree. C.

　　18. The method of claim 7 wherein the ratio of the halogenated organic solvent and the second organic solvent is 1:1.

　　19. The method of claim 6 wherein the temperature of the solution is lowered to within the range of from -20.degree. C. to -10.degree. C. for from 2 to about 3 hours.

　　20. The method of claim 6 wherein the recrystallized lutein is dried in vacuo at 50.degree. C. for 3 days.
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摘要信息如下，如果需要详细内容可以在谷歌中找“google patents”，点击大概是第一个链接进去。输入5,382,714即可找到了。 United States Patent 5,382,714 Khachik January 17, 1995 Process for isolation, purification, and recrystallization of lutein from saponified marigold oleoresin and uses thereof Abstract A method of isolating, purifying and recrystallizing substantially pure lutein, preferably from saponified marigold oleoresin in its pure free form, apart from chemical impurities and other carotenoids. Lutein may be used as an analytical standard or in cancer prevention trials and as a safe and effective color additive for human food. Inventors: Khachik; Frederick (Beltsville, MD) Assignee: The Catholic University of America (Washington, DC) Appl. No.: 08/210,009 Filed: March 17, 1994