spike-in control翻译

spike-in control翻译

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An RNA spike-in is an RNA transcript used to calibrate measurements in a DNA microarray experiment. Each spike-in is designed to hybridize with a specific control probe on the target array. Manufacturers of commercially available microarrays typically offer companion RNA spike-ins "kits".[1][2][3][4]
Known amounts of RNA spike-ins are mixed with the experiment sample during preparation. Subsequently the measured degree of hybridization between the spike-ins and the control probes is used to normalize the hybridization measurements of the sample RNA.
RNA spike-in是用于对DNA微阵列试验进行校正的DNA转录物，这种spike-in可以和标靶阵列的特异性控制（对照）探针进行杂交。商业化微阵列扫描仪的商家一般会同时提供的RNA spike-in试剂盒。
制备样品时，将已知量的RNA spike-in和试验样本混合，然后检测spike-in和控制（对照）探针的杂交程度，用这样的数据对来样品RNA的杂交水平数值进行校正。